Bioanalytical Core Laboratory

The Georgetown University Bioanalytical Center {BAC} is chromatography lab located on the ground level of the Preclinical Sciences Building and occupies approximately 1500 square feet in rooms GD1 and GD3. The BAC contains a core laboratory that is funded as part of the Georgetown University Clinical Research Center but is also available to investigators at the Medical Center, the University, as well as outside clients, on a fee-for-service basis. The laboratory is dedicated to the development, validation and application of bioanalytical methods in support of clinical, pharmacokinetic and pharmacogenetic studies as well as basic pharmacological research.

The staff consists of experienced laboratory scientists, all of whom are capable of performing the following services:

• Method development for established and experimental drugs or other analysis of interest.
• Method validation
• Sample analysis from clinical studies
• Confirmation of mass and/or purity of products resulting from synthesis or in-vitro metabolism studies
• Chiral assays
• Separation and collection of chiral enantiomers
• Immunoassays
• Use of HPLC as sample clean-up for immuno-assays
• Liquid chromatograph with mass spectroscopy
• High performace liquid chromatography with UV and fluorescence detection
• Gas chromatography with nitrogen, phosphorus, and flame ionization detection
• Capillary electrophoresis with UV and laser-induced detection
  

The laboratory maintains the equipment listed below:

• 5 HPLC systems with UV, fluorescence and electrochemical detectors (ThermoSeparations/Agilent)
• 2 Capillary electrophoresis (CE) systems with UV detectors (ABI/PE)
• 1 CE system with UV and laser-induced florescence (LIF) detectors (Biorad)
• API-3000 Mass Spectrometer (sciex) (Applied Biosystems)
• API-4000 Mass Spectrometer
• IMMULITE by Diagnostic Products Inc

Some of the systems are fully automated with autosamplers and on-line computer-based data collection. All necessary support equipment for the storage {three -80 °C and one -20 °C freezers} and preparation {balances, pH meters, centrifuges, solid phase extraction apparatus, etc} of clinical samples are contained within the laboratory.